Recombinant Cytokeratin 19 anticorps (CF®594)

N° du produit ABIN7656107

L’anticorps Souris Monoclonal anti-Cytokeratin 19 a été validé pour WB, IHC, FACS et IHC (fp). Il convient pour détecter Cytokeratin 19 dans des échantillons de Humain.

Aperçu rapide pour Recombinant Cytokeratin 19 anticorps (CF®594) (ABIN7656107)

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 19 est conjugé à/à la CF®594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RKRT19-799

Détail du produit anti-Cytokeratin 19 anticorps (CF®594)

Fonction: Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) (rKRT19/799), CF594 conjugate

Attributs du produit: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human KRT19 protein

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat or ferret. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cytokeratin 19

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Autre désignation: Keratin 19

Sujet:

Synonyms: k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19

Gene Symbol: KRT19

Tissue Expression: Epithelial cells

Poids moléculaire: 40 kDa

ID gène: 3880

UniProt: P08727